|Synonyms||GelMA Bioink, GelMA Lyophilizate Bioink, Methacrylated Gelatin Bioink, Gelatin Methacrylate Bioink, Gelatin Methacryloyl Bioink||Sterility||Sterile|
|Endotoxin level||<50 EU/mL|
|Cell viability||≥85% fibroblast, osteoblast and mesenchymal stem cells|
|pH||7.3 – 7.4|
|Degree of methacrylation||>80% (GelMA High)
50-60% (GelMA Middle)
Gelatin methacrylate (GelMA) bioinks developed by AdBioInk Biosystem Corp. are prepared by using two different photoinitiators, Irgacure2959 and LAP. Cell adhesion and cell encapsulation experiments were performed against NIH-3T3 cell line. In cell viability assays, Live/Dead kit was used. It was observed that the cell viabilities in UV or visible light crosslinked and cell-encapsulated GelMA bioinks were over 95%.
Cell morphologies in and onto the crosslinked-GelMA bioinks were analyzed by DAPI-ACTIN staining. Cells seeded on GelMA bioinks took their cell morphology on day 1 and proliferated by day 7, showing over 90% confluence on bioink. Encapsulated cells appear to begin to show cytoplasmic protrusions at day 7.