Kappa-Carrageenan Bioink

$79,00$219,00

Kappa-carrageenan bioink is the first ever commercialized kappa-carrageenan bioink for 3D bioprinting. Carrageenan is a hydrophilic polymer extracted from algae and is used as a gelling agent and emulsifying agent. Among different types of carrageenan, Kappa-carrageenan (κCA), a natural linear water-soluble polysaccharide with one sulphated group per disaccharide (25 to 30% ester sulfate content), is one of the appropriate biomaterial in tissue engineering.  Kappa-carrageenan bioink has been seen as a potential candidate for this area in injectable form, due to its gelation properties, mechanical strength and resemblance to chondroitin-4-sulfate anddermatan sulfate, which are the major components of native extracellular matrices (ECM) called glycosaminoglycans (GAGs).

Kappa-carrageenan bioink is a thixotropic polymer and can be converted into hydrogel form via ionic crosslinking method. However, these crosslinking methods offer hydrogels that have low stability in physiologic conditions. In order to overcome this drawback, Kappa-carrageenan methacrylating to synthesis KapMA with photopolymerization potential.

Kappa-carrageenan bioink has a characteristic of tunable viscosity depending on concentration, temperature, the presence of ions and molecular weight and has properties of high strength, nontoxic and cellular compatibility. As its shear-thinning and thermo-reversible properties are suitable for bioinks, Kappa-carrageenan could been chosen as a bioink material for 3D extrusion-based bioprinting.

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Description

Sterility Sterile
Endotoxin level <50 EU/mL
Cell viability ≥85% mesenchymal stem cells and osteoblast cells
pH 7.3 – 7.4
Degree of methacrylation >80% (GelMA High)

50-60% (GelMA Middle)

Form Gel

Kappa-carrageenan bioink (KapMA) was developed by AdBioink and cell experiments were performed with the fibroblast cell NIH 3T3. Cell adhesion and encapsulation experiments of Kappa-carrageenan bioinks that photo-crosslinked in 2 different ways with UV and visible light were performed. Cell morphologies were examined on the 1st and 7th days by DAPI-Actin staining. Cells adhered to Kappa carrageenan bioinks, proliferated and were able to preserve their cell morphology. It is seen that the encapsulated cells begin to form cytoplasmic extensions on the 7th day. According to the Live/Dead staining results in which the cell viability was examined, it was seen that the cell viability was over 95% and the encapsulated cells preserved their viability for 7 days.

KapMA Bioink Cell Adhesion, KapMA Bioink Cell Viability, KapMA Bioink Cell Morphology, Adbioink, Bioink

Additional information

Volume

3ml, 3x3ml

Photoinitiator type

Irgacure 2959, LAP

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